Laboratories

Laboratory of Molecular Genetics

[head prof. Antonio Russo]

Biomolecular characterization of rare and solid hereditary tumors (breast, colon, NET, GIST).
Analysis of gene expression profiles and miRNAs in vitro and / or in tissue and blood samples. Analysis of gene copy number (CNV) variation in major solid tumors.
Translational research studies aimed at new therapeutic strategies (pharmacogenetics).
Liquid biopsy as a dynamic tool for assessing the response to treatment and the onset of resistance in patients with non-small cell lung cancer (NSCLC)
Liquid biopsy for the evaluation of minimal residual disease in patients with resectable colorectal cancer
Molecular analysis of oncohematological pathologies (myeloproliferative disorders and chronic myeloid leukemias).
In vitro experimentation on tumor cell cultures.
In vitro microRNA function analysis.
Isolation and preparation of primary cell cultures.
Determination of new possible biomarkers in the main biological samples.
Analysis of BRCA1 / 2 genes for the identification of genetic breast and ovarian syndrome (HBOC, hereditary breast and ovarian cancer)
Analysis of BRCA1 / 2 genes in patients with advanced ovarian and peritoneum cancer for treatment with PARP inhibitor drugs
Analysis of immune checkpoints in the peripheral blood of cancer patients.

Next Generation Sequencing platform, Ion S5 (ThemoFisher Scientific)
QuantStudio 3D (ThemoFisher Scientific)
Idylla (Biocartis)
ABI PRISM 3100 Genetic Analyzer, Applied Biosystems. Use: Apparatus for genetic sequences.
ABI PRISM 3130XL Genetic Analyzer, Applied Biosystems. Use: Apparatus for genetic sequences and analysis of DNA fragments.
Real-Time 7900HT Sequence detector with SDS software, Applied Biosystems. Usage: for gene expression analysis in quantitative and qualitative analysis for SNPs
Affymetrix microarray platform. Usage: for gene expression analysis
Agilent 2100 Bioanalyzer. Usage: quantitative and qualitative analysis of nucleic acids and proteins, gels.

Purification, propagation of stem cells from different types of cancer such as: Colon, Breast Thyroid, Ovary, Prostate, Lung and Glioblastoma;
Identification of the phenotypic and molecular characteristics of cancer stem cells;
Tumor-specific molecular analysis of molecules having a key role in the process of tumorigenesis and metastagenesis;
Understanding of the mechanisms that control tumor formation and progression, chemoresistance and metastasis formation;
Study of the mechanisms of resistance to common antitumor therapies of cancer stem cells;
Development of a new technological platform for the non-invasive treatment of oncological diseases based on the use of focused ultrasound;
Development of new anticancer therapeutic strategies based on genomics and functional proteomics investigations of cancer stem cells: identification of effective drugs targeting cancer stem cells;
Development of experimental models of tumor stem cell xenograft for the testing of new drugs on human-derived tumors;
Therapeutic implications of cancer stem cells from adipose tissue for the regeneration of bone, cartilage and muscle tissue.

[laboratory technician dr. Anna Barbara Di Stefano]

Study of a new population of adipose stem cell spheroids (SASCs), molecular characterization: genomic and proteomic analysis.
Study of the immunomodulatory role of adipose stem cell spheroids in vascularized composite transplants: induction of immunological tolerance and in vivo monitoring.
Analysis and comparison between the different anatomical sites of sampling and extraction techniques of stem cells from adipose tissue
Isolation of epithelial tumor stem cells for the purpose of testing new anticancer therapies in vitro.
Development of new molecular technologies through the use of focused ultrasound.
Isolation and preparation of primary cell cultures.

Class III isolator Use: manipulation and preparation of cell cultures.
ChemiDoc Imaging System, Use: scientific image analysis system equipped with internal computer, Image Lab Touch software and StainFree technology.
Leica DM IL LED Fluo with camera.
Direct-Q® 3 UV Kit IT. Use: modular equipment for the production of pure and ultrapure water.
Qubit ™ 4 Fluorometer Use: for the quantitative analysis of DNA, RNA and proteins.